Inverted fluorescence microscope

LMI EA-Prime

A research-grade inverted fluorescence microscope for cell culture, live-cell fluorescence, transfection studies, protein transfer and detailed observation of cell growth.

  • 22 mm field with infinity colour-corrected optics
  • Professional semi-apochromatic fluorescence objectives
  • Four filter-cube positions: three fluorescence and one bright field
  • Bright field, dark field, phase contrast and polarization
View specifications
LMI EA-Prime inverted fluorescence microscope
Optical systemInfinity corrected
Field of view22 mm
Fluorescence pathsFour filter cubes
IlluminationLED or halogen

Technical information

EA-Prime specifications

Long-working-distance optics and configurable culture-vessel holders support a broad range of live-cell and fluorescence workflows.

Model
LMI EA-Prime microscopeEA-Prime Inverted
Optical system
Infinity-corrected, anti-fungal optical system.
Eyepieces
EA-Prime eyepiecesPL10X/22 mm and PL15X/16 mm high eye-point plan eyepieces.
Objectives
EA-Prime objectives

Infinity LWD plan achromatic: LWDPL4X/0.10/WD22; 10X/0.25/WD7.94; 20X/0.40/WD7.66; 40X/0.60/WD3.71; 60X/0.70/WD2.50.

Infinity LWD phase contrast: 10X/0.25/WD7.94; 20X/0.40/WD7.66; 40X/0.60/WD3.71.

Infinity LWD plan fluorescence: 4X/0.13/WD18.52; 10X/0.30/WD7.11; 20X/0.45/WD5.91; 40X/0.65/WD1.61; 60X/0.75/WD1.04.

Infinity LWD fluorescence phase contrast: 20X/0.45/WD5.91 and 40X/0.65/WD1.61.

Viewing head
45° Siedentopf binocular or trinocular head; 54–75 mm or 50–75 mm interpupillary adjustment; ±5 diopter adjustment. Light splits: binocular 100%, trinocular 100%, or binocular 80% / trinocular 20%.
Nosepiece
Quintuple nosepiece.
Stage and holders

160 × 250 mm platform with mechanical stage, 120 × 78 mm movement, extension plate, glass stage and slotted metal stage.

Holders for specimen slides, Ø54 mm, Ø65 mm Terasaki and Ø35 mm tissue-culture vessels.

Focus adjustment
EA-Prime focus controlsAll-metal die-cast body with precision rack-and-pinion transmission, 25 mm coarse range, tension adjustment, upper limit stop and 0.002 mm fine precision.
Microscope body
EA-Prime microscope bodyCoaxial focus system with 25 mm coarse travel, 0.002 mm fine precision, tension adjustment and built-in 90–240 V wide-voltage transformer.
Reflected fluorescence
EA-Prime reflected fluorescence system

Köhler reflected illuminator with iris field diaphragm and centre adjustment.

100 W mercury lamp housing with adjustable centre and focus, digital 90–245 V AC supply, centring objective, UV1/B1/G1/violet band-pass filter cubes and 30ND25 attenuation pad.

Transmitted illumination
EA-Prime transmitted illumination

Critical illumination with 6 V / 30 W halogen or 5 W LED, preset centre, adjustable brightness and iris aperture diaphragm.

Köhler illumination option adds centre adjustment and field diaphragm.

N.A. 0.3 LWD condenser, WD72 mm; condenser stand can swing out to increase working distance to 150 mm. LBD colour-temperature and IF550 green contrast filters included.

Phase contrast
EA-Prime phase-contrast accessories

30 mm centring telescope.

EASL or SL1 10X–40X phase-contrast slider with centre adjustment.

CCD adapter
C-mount adapter.
Fluorescence filters
EA-Prime fluorescence filtersStandard UV, V, B and G fluorescence filters. Four filter cubes can be fitted and custom filter sets are available.
Camera and software
Compatible options are available from the LMI camera and software range.

Planning an EA-Prime live-cell imaging system?

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