Inverted fluorescence microscope
LMI EA-100i
An advanced inverted system for convenient cell-culture observation, live-cell fluorescence, transfection studies, protein transfer and detailed analysis of cell growth.
- Infrared ECO system automatically controls illumination power
- 22 mm field with high-contrast infinity-corrected optics
- Servo-motor switching for four fluorescence filter paths
- Bright field, dark field, phase contrast and polarization; DIC upgradable
Technical information
EA-100i specifications
Long-working-distance optics and configurable culture-vessel holders support a broad range of live-cell and fluorescence workflows.
EA-100i
PL10X/22 mm and PL15X/16 mm high eye-point plan eyepieces.
Infinity LWD plan achromatic: LWDPL4X/0.10/WD22; 10X/0.25/WD7.94; 20X/0.40/WD7.66; 40X/0.60/WD3.71; 60X/0.70/WD2.50.
Infinity LWD phase contrast: 10X/0.25/WD7.94; 20X/0.40/WD7.66; 40X/0.60/WD3.71.
Infinity LWD plan fluorescence: 4X/0.13/WD18.52; 10X/0.30/WD7.11; 20X/0.45/WD5.91; 40X/0.65/WD1.61; 60X/0.75/WD1.04.
Infinity LWD fluorescence phase contrast: 20X/0.45/WD5.91 and 40X/0.65/WD1.61.
215 × 250 mm fixed stage; attachable mechanical stage with 120 × 80 mm movement; extension plate, slide holder, Petri-dish holder, Terasaki holder, metal plate and glass plate.
Terasaki holder also accepts Ø65 mm tissue-culture vessels; dedicated holder available for Ø35 mm vessels.
Biological frame with coaxial focus, 9 mm coarse range (6.5 mm upward and 2.5 mm downward), 0.002 mm fine precision, upper limit and tension adjustment.
Coaxial focus system with 25 mm coarse travel, 0.002 mm fine precision, tension adjustment and built-in 90–240 V wide-voltage transformer.
Köhler reflected illuminator with iris field diaphragm and centre adjustment.
LED fluorescence lamp housing with servo-motor four-band switching: UV1 385 nm, B1 470 nm, G1 560 nm and violet; includes 30ND25 attenuation pad.
Critical illumination with 6 V / 30 W halogen or 5 W LED, preset centre, adjustable brightness and iris aperture diaphragm.
Köhler illumination option adds centre adjustment and field diaphragm.
N.A. 0.3 LWD condenser, WD72 mm; condenser stand can swing out to increase working distance to 150 mm. LBD colour-temperature and IF550 green contrast filters included.
30 mm centring telescope.
EASL or SL1 10X–40X phase-contrast slider with centre adjustment.
Planning an EA-100i live-cell imaging system?
Request a quotation| Optical System | Infinity color corrected optical system |
| Viewing Head | 45° inclined trinocular head, 360° rotatable, inter-pupillary range: 50-75mm, fixed splitting ratio = 5:5 |
| 45° inclined trinocular head, 360° rotatable, inter-pupillary range: 50-75mm, splitting ratio R:T = 100:0 or 0:100 |
| Eyepiece | High eye-point wide field plan eyepiece PL10X/22mm, with adjustable diopter, micrometer attachable |
| High eye-point wide field plan eyepiece PL15X/16mm, with adjustable diopter |
| Objective | Infinity plan achromatic objectives 4X/10X/20X/40X/60X |
| Infinity plan phase contrast objectives 4X/10X/20X/40X |
| Infinity plan fluorescence objectives 4X/10X/20X/40X/60X |
| Infinity plan phase contrast and fluorescence objectives 20X/40X |
| Nosepiece | Quintuple nosepiece |
| Quadruple nosepiece |
| Frame | Biological frame with coaxial focus system, coarse range: 9mm (6.5mm up and 2.5 down), fine precision: 0.002mm. With upper limit and tension adjustment. External wide voltage transformer, 100V-240V input and 12V5A output. With ECO function. |
| Fluorescent frame (transflection), Biological frame with coaxial focus system, coarse range: 9mm (6.5mm up and 2.5 down), fine precision: 0.002mm. With upper limit and tension adjustment. Max. 4 LED fluorescent filters. External wide voltage transformer, 100V-240V input and 12V5A output; 5W LED with light indicator, intensity adjustable. With infrared induction. With ECO function. |
| Transmitted Illumination | Transmitted light source with cold/warm color options, cool color temperature 5000K, warm color temperature 3000K. |
| Fluorescent Illumination | Kolar Lighting, 5W LED contains three sets of LED light sources of 385nm, 470nm, and 560nm, equipped with UV1, B1, and G1 fluorescence color filter sets respectively. The predetermined center, the servo motor controls the light source to automatically switch with the color filter set. |
| Stage | 215mm × 250mm fixed stage; mechanical stage attachable, moving range: 120mm × 80mm; extension plate; slide holder; Petri dish holder; Terasaki holder; metal plate; glass plate. |
| Condenser | NA0.3 long working distance condenser, demountable. |
| Camera Adapter | 0.35X, 0.5X, 0.65X, 1X C-mount adapter |
| Others | Color temperature change filter, green interference filter, infrared eliminate filter |